691. A Light-Inducible CRISPR/Cas9 System for Control of Endogenous Gene Activation

A light-inducible CRISPR/Cas9 system for control of endogenous gene activation

Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively...

RiboTALE: A modular, inducible system for accurate gene expression control

A limiting factor in synthetic gene circuit design is the number of independent control elements that can be combined together in a single system. Here, we present RiboTALEs, a new class of inducible repressors that combine the specificity of TALEs with the ability of riboswitches to recognize exogenous signals and differentially control protein abundance. We demonstrate the capacity of RiboTAL...

Light-Inducible Spatiotemporal Control of Gene Activation by Customizable Zinc Finger Transcription Factors

Advanced gene regulatory systems are necessary for scientific research, synthetic biology, and gene-based medicine. An ideal system would allow facile spatiotemporal manipulation of gene expression within a cell population that is tunable, reversible, repeatable, and can be targeted to diverse DNA sequences. To meet these criteria, a gene regulation system was engineered that combines light-sen...

An inducible CRISPR-ON system for controllable gene activation

فرآیند کنترل در سیستم کامپیوتری تهویه مطبوع ‏‎airwasher environmental control system for intelligent buildings‎‏

در این پایان نامه با استفاده از تکنولوژی روز و امکانات داخل کشور طریقه اتوماسیون سیستم تاسیساتی یک کارخانه نساجی (کارخانه نساجی اطلس پود) همراه با منوهای مربوطه و توضیحات کامل برنامه نویسی و طراحی بلوک های برنامه و سایر قسمتهای برنامه ارائه گشته است. علاوه بر این، برنامه با نمونه آمریکایی آن مقایسه و مزیت های آن نسبت به آمریکایی توضیح داده شده است. همچنین تمامی بلوک های برنامه بصورت شماتیکی بطو...